Any researcher who works in a cell culture hood needs some sort of cell counting solution whether to determine cell concentration prior to cell passage or to assess cell viability following drug treatment.
Bio rad cell counter trypan blue.
When using trypan blue the counter automatically takes into account the 1 1 dilution.
If you own the tc10 automated cell counter please visit the accessories tab on this page to order additional kits reagents and accessories for your.
This test is often performed while counting cells with the hemocytometer during routine subculturing but can be performed any time cell viability needs to be determined quickly and accurately.
450021 and 10 µl of this cell trypan blue suspension was added into a cell counting slide bio rad.
Trypan blue staining is the method employed by the countess family of automated cell counters and this 0 4 trypan blue solution is the correct concentration for use on these instruments.
For each protein that is produced using this method considerable attention must be paid to the density of the cell cultures at various stages in the protocol.
Precipitates are quite common in trypan blue and typically increase with storage time and freezing.
View product view product specs our lab uses insect cell cultures to produce recombinant mammalian proteins.
Cell counters as the name implies are tools for counting live and or dead cells in a culture.
Of course of dead cells is cell type dependent.
Cell viability was determined by the result analyzed by a tc20 automated cell counter bio rad.
From the results screen you can view an image of the cells use the built in dilution calculator or view a histogram of cell sizes and when using trypan blue the spread of cell sizes versus viability.
Tc 10 automated cell counter catalog number.
Obtain a total cell count without trypan blue or total and live cell counts with trypan blue in 30 seconds.
Trypan blue solution 0 4 is routinely used as a cell stain to assess cell viability using the dye exclusion test.
5 x 1 5 ml sufficient for 750 counts 10 µl count 0 4 in 0 81 sodium chloride and 0 06 potassium phosphate dibasic solution sterile filtered for use with tc10 or tc20 automated cell counter.
Of course trypan blue assay is not sufficient to evaluate dead cells it s just an indication of cell death.
Home life science research bio rad products trypan blue trypan blue 1450021.
