Bio rad provides three types of molecular rulers.
Bio rad 20 bp ladder.
Molecular mass rulers bio rad precision molecular mass rulers dna ladders allow accurate dna quantitation in gels making them ideal for densitometry or image analysis.
Bio rad molecular rulers are dna standards with precisely defined size intervals between bands dna ladders for simplified estimation of the length of nucleic acids separated on agarose gels.
Standard ez load and amplisize rulers.
Compare 20 bp ladders from leading suppliers on biocompare.
Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
50 bp dna ladder is stable for at least 3 months at 4 c.
Standard ez load and amplisize rulers.
General description sigma s pcr 20 bp low ladder contains 50 dsdna fragments from 50 1 000 bases evenly spaced in 20 bp repeats.
For long term storage store at 20 c.
200 μl 0 2 μg μl dna standard 500 8 000 bp 16 bands in 500 bp increments 100 applications.
Dna may be denatured if diluted in dh 2 o.
Use 1 0 5 0 µl for full length gels 16 20 cm to yield 100 or up to 500.
1x gel loading dye purple no sds.
If samples need to be diluted use te or other buffer of minimal ionic strength.
The size markers used were a 100 bp ladder ez load 100 pb pcr molecular ruler biorad marnes la coquette france or 20 bp ladder ez load 20 pb molecular ruler biorad marnes la coquette france according to the tandem repeat unit length.
250 μl 0 2 μg μl dna standard 20 1 000 bp 50 bands in 20 bp increments 100 applications.
Browse bio rad s wide range of quality prestained and unstained protein standards.
We recommend loading 0 5 1 0 µg of 50 bp dna ladder diluted in sample buffer.
Or 0 5 2 5 µl for silver staining up to 1 000 applications.
500 μl 0 05 μg μl ready to load dna standard 100 1 000 bp 10 bands includes 1 ml 5x nucleic acid sample buffer 100 applications.
Gel images were managed using the bionumerics software package version 4 0 applied maths sint.
